Inertsil ods4, ods3, odssp, ods2 reversedphase methanolwater columns 5. Hplc columns hplc column selectivity measurements of more. Inertsil ods 4 minimizes the dewetting phenomenon while maintaining the same retention characteristics to that of common ods columns. The offered products are widely demanded for their features like safe usage, easy to rinse and reasonable prices. Inertsil hplc columns for reversed phase separation 2.
Figure 11 shows the application of this column to the analysis of cytochrome c tryptic. Inertsil ods 3 has a relatively strong retentivity compared to commercially available ods columns. Inertsil ods4 minimizes the dewetting phenomenon while maintaining the same retention characteristics to that of common ods columns. All standard geometries available most from stock priced competitively gl sciences inertsil hplc columns offer a significant advantage in the analysis of basic molecules, amines and accidic compunds. Ods columns find a wide range of applications due to their high theoretical plate number and rapid equilibration. Conditions chosen based on simplicity of mobile phase for nonionic. Their low cost makes them the most widely used columns for hplc. Inertsil ods 4, ods 3, ods sp, ods 2 reversedphase methanolwater columns 5. Inertsil ods3 users can, therefore, readily change to the new, validated inertsil ods3v columns with complete confidence. Inertsil ods 4 a musthave column in your laboratory reversed phase ods and c8 columns comprise the vast majority of columns used in the hplc separation industry. Specifications of various inertsil ods columns surface area pore size purity carbon loading bondedphase structure endcapping inertsil ods4 450 m2g 100 a 99,999% 11 % monomeric inertsil ods3 450 m2g 100 a 99,999% 15 % monomeric inertsil odsp 450 m2g 100 a 99,999% 29 % polymeric no. Comparison guide to c18 reversed phase hplc columns. Inertsil ods3v is a highly efficient, validated hplc column.
Phenomenex uhplc, hplc, spe, gc leader in analytical. Inertsil wp300 300a series wp300c18, c8, c4, diol, sil inertsil odshl 100a series. Hplc column comparison screening study for reversed. A 100% water mobile phase hplcpda analysis of meamine. Figure 11 shows the application of this column to the analysis of cytochrome c tryptic digest. In addition, the introduction of higher surface area silica. A 100% water mobile phase hplcpda analysis of melamine and related analogues. Inertsil sulfa c18 inertsil sulfa c18 3 and 5m is designed for the analysis of sulpha drugs. Hypersil stationary phases are recognized as an industry standard in hplc. A 100% water mobile phase hplc pda analysis of melamine and related analogues. View all luna hplc column part numbers luna c18 2 is a recommended alternative to. Thermo scientific hypersil ods2 c18 hplc columns are an alternative to older column brands commonly referenced in validated methods.
The original 2series inertsil phases, including silica, c4, c8, ods2 and phenyl, created the. From the first generation inertsil ods to the latest inertsustain series, the inertsil series columns are widely used around the world ensuring column quality and performance anywhere in the world. Inertsil ods3 analytical columns products gl sciences. Inertsil ods4 column name functional group inertsustain c18 inherits the advantages of all the current inertsil hplc columns, but now can be used for wide ph analysis with consistent performance. Inertsil ods3v series product data 1020 gl sciences. Inertsil ods inertsil ods series 1,2,3 isco c18 jsphere odsh80 jsphere odsl80 jsphere odsm80 jupiter c18 kinetex c18 kromasil c18 lichrospher pah lichrosorb rp18 lichrospher 100 rp18 lichrospher rp18e luna c18 luna c182 diazem c18 60a series 1,2,3 diazem c18 series ec diazem c18 dualzone discovery c18 discovery hs c18. Inertsil odssprint inertsil peptide c18 the inertsil peptide c18 column is speci cally suited to the analysis of peptide digests. Ods 80a was originally introduced to provide a higher surface area alternative to ods 2, but with the same inertness and basedeactivated properties. Therefore, inertsil ods 4 can be used at ease under 100% aqueous eluents. Inertsil c4 inertsil wp300 c4 microsorb c4 bondesil silica inertsil econo prepsil generik silica generik c8amino protec c8 chromegabond c2e gpc2 nucleosil c2 l31 l32 l33 l34 l35 l36 usp listing of hplc columns l packingbrand name brand name l37 l38 l39 l40 l41 l43 l44 l51 l48 l49 l52 l53 l54 l55 l56 l57 l58 l59 l60 l61 packing having the. These columns are optimized for ultrafast hplc separations with more resolving power for small molecules.
Inertsil 4 series columns are the most recent addition to the inertsil line and the most inert columns available. Shop online for a wide selection of thermo scientific hypersil ods c18 columns use as a general purpose c18 phase for acidic and neutral analytes. Inertsil c84 is an octyl group c8 bonded column providing the same separation pattern and extreme inertness to any type of compounds just like inertsil ods4, which enables rapid analysis of highly hydrophobic compounds delivering symmetric peaks and easy method transfer from. Specifically qualified hplc columns for glpgmp compliance. Hplc column technical guide introduction specifications shipping solvent mobile phase. Graduate school of human life science, osaka city university, osaka, japan. Specifically qualified hplc columns for glpgmp compliance validation inertsil ods3v offers all of the outstanding chromatographic benefits of inertsil. Inertsil ods columns are general purpose, reversed phase c18 columns. We recommend inertsustain c18 or inertsil ods4 columns for all new method development. Inertsil odsep c18 embedded polar functional groups is compatible with 100% aqueous eluents. Inertsil ods sprint inertsil peptide c18 the inertsil peptide c18 column is speci cally suited to the analysis of peptide digests. Hplc column selection a comparison of reversedphase columns based on the widely accepted work and data of drs.
Inertsil ods4 and c84 columns also show excellent stability during the analysis of strong metal. Luna c18 ace superc18 ymcpack odsam symmetry c18 kromasil c18 lichrosorb rp18 nucleodur c18 nucleosil c18 inertsil ods2 c18 acclaim c18. The same high performance characteristics found in inertsil ods 3 have been incorporated in the manufacture of inertsil ods 3v. The same high performance characteristics found in inertsil ods3 have been incorporated in the manufacture of inertsil ods3v. Classical phases tm hypersil thermo electron corporation. However, a variety of problems may arise while using these columns. Inertsil ods3 and ods3v capital hplc limited hplc columns. Octadecylsilica ods is the industry standard packing material for hplc applications. Hplc column comparison screening study for reversed phase columns. Inertsil hplc column catalog inertsil hplc column catalog.
Sd analytical, was established in the year of 2014. H 1 h 2 is the difference between the hydrophobicity parameters of columns 1 and 2 s 1 s 2 is the difference between the steric parameters of columns 1 and 2 a 1 a 2 is the difference between the hydrogen bond acidity parameters of columns 1 and 2 b 1 b 2 is the difference between the hydrogen bond basicity parameters of columns 1. The typical particle size of traditional hplc columns is 5m, but in the past, 3. Hl recognizes even slight steric differences achieving complete baseline separation while other conventional c18 column fails. Inertsil diol enable sugars analysis with a long column. The inertsil ods3 series represents a major advance in performance over the original inertsil 2series. Hplc columns c18 amide c4 c8 sec hilic phenyl t3 shield. Gl sciences hplc columns can tolerate up to pressures shown below. Inertsil ods sprint operates at low back pressures, even at high ow rates and shows excellent robustness and reproducibility. Choose thermo scientific hypersil ods c18 hplc columns for a general purpose c18 phase to use in a broad range of applications. Inertsil ods 5 100 350 14 yes no inertsil ods3 5 100 450 15 yes yes inertsil ods2 5 150 320 18. Cortecs columns solidcore particle technology in 1. Inertsil 3series phases are based on a purer, higher surface area silica which is specially manufactured to provide maximum bonded phase coverage.
Lc columns and consumables ed kim application engineer february, 2008. Hypersil ods2 c18 columns thermo fisher scientific. The longawaited validated inertsil ods4v has now been added to our product lineup. Inertsil ods columns are general purpose, reversed phase c18 columns available in 5. Inertsil ods 3 is still gl sciences most popular phase and continues to be used widely and reliably for long established methods in pharmaceutical, and contract research labs. As shown in figure 1, inertsil ods 3 has a relatively strong retentivity compared to commercially available ods columns. The original 2series inertsil phases, including silica, c4, c8, ods 2 and phenyl, created the high purity silica revolution in the late 1980s. A 100% water mobile phase hplcpda analysis of meamine and. Reversed phase hplc columns comparison data on commonly used c18 phases analytical inc. Reversedphase c18 columns ods versatile hybrid silica based hplc column ymctriart c18 ymctriart c18 is a hybrid silica based ods column emphasizing durability, low operating pressure, and excellent performance. Specifications of various inertsil ods columns surface area pore size purity carbon loading bondedphase structure endcapping inertsil ods 4 450 m2g 100 a 99,999% 11 % monomeric inertsil ods 3 450 m2g 100 a 99,999% 15 % monomeric inertsil ods p 450 m2g 100 a 99,999% 29 % polymeric no. Inertsil hplc packings are subject to a rigorous array of qc tests in a iso9001 compliant facility, with special emphasis on reagent purity, raw material traceability, and consistency in raw materials and finished. Proven as an effective analytical tool, the classical hypersil phases are well established and referenced in many hplc methods worldwide.
Inertsiltm ods4v 3 m, 5 m manufacturers validation certificate sheet inertsil ods4 columns have proven superior worldwide for analysis of strong pharmaceutical bases, acids, chelating compounds, and zwitterions. The result is a series of columns which provide excellent peak shapes using simple eluents. Ymctriart c18 is a hybrid silica based ods column emphasizing durability, low operating pressure, and excellent performance. Hplc columns c18 amide c4 c8 sec hilic phenyl t3 shield rp18. Ods columns are used for reversephase chromatography. Ymctriart c18 has been developed and is manufactured using stateoftheart technologies that define materials, granulation.
Inertsil inods3150dea 0 gbp inods3150d 5547593 5547596 502001900 5547594 inods3250d 502001921 5547588 5547595 5547585 5547587 502005044 5547586 inods2150a. In general, elution time of inertsil ods 4 is shorter than inertsustain c18 and ods 3. We are manufacturer, exporter, wholesaler, supplier, distributor and importer of hplc columns, vials and filter paper. Typically chromatographers must choose hplc columns by comparing the surface area and carbon load data. A comparison of alltech c18 and c8 columns alltech has developed a method for choosing c18 and c8 columns based on peak capacities and column selectivity of polar and nonpolar compounds. Inertsil ods 3 is still gl sciences most popular phase and continues to be used world widely and reliably for long established methods in pharmaceutical, and contract research labs. Inertsil ods 4 column name functional group inertsustain c18 inherits the advantages of all the current inertsil hplc columns, but now can be used for wide ph analysis with consistent performance.
We use cookies to improve your experience and our website service. Agilent technologies zorbax sbc18, waters symmetry c18, sigmaaldrich biotechnology supelcosil abz and gl sciences inertsil ods 2. Hypersil ods 5 120 170 10 yes no inertsil ods 5 100 350 14 yes no inertsil ods3 5 100 450 15 yes yes inertsil ods2 5 150 320 18. Inertsil odsp high steric selectivity l1 3, 5 10 450 inertsil odsep a polar functional group embedded l1 5 10 450 inertsil wp300 c18 analysis of high molecules l1 5 30 150 inertsil ods80a elute low molecule with a sharp peak shape l1 5 8 450 inertsil ods2 ultra pure silica gel is used l1 5 15 320 inertsil ods inertness 1st generation l1 5. Phenomenex is a global manufacturer of uhplc, hplc columns, gc columns, sample preparation products and chromatography accessories and consumables. Furthermore, the packing material that achieves a stable analysis using 100% aqueous eluents provides another.
This series features a variety of chemical bond bases such as c18,c8,nh2,cn, phenyl, hilic, and amide, and is able to meet a wide array of. Inertsil ods p high steric selectivity l1 3, 5 10 450 inertsil ods ep a polar functional group embedded l1 5 10 450 inertsil wp300 c18 analysis of high molecules l1 5 30 150 inertsil ods 80a elute low molecule with a sharp peak shape l1 5 8 450 inertsil ods 2 ultra pure silica gel is used l1 5 15 320 inertsil ods inertness 1st generation l1 5. Therefore, inertsil ods4 can be used at ease under 100% aqueous eluents. Inertsil ods 3 users can, therefore, readily change to the new, validated inertsil ods 3v columns with complete confidence. Strong retentivity, lower column backpressure, very inert.
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